Transcriptome profiling of tobacco under water deficit conditions

نویسندگان

  • Roel C. Rabara
  • Prateek Tripathi
  • Mani Kant Choudhary
  • Michael P. Timko
  • Qingxi J. Shen
  • Paul J. Rushton
چکیده

Drought is one of the limiting environmental factors that affect crop production. Understanding the molecular basis of how plants respond to this water deficit stress is key to developing drought tolerant crops. In this study we generated time course-based transcriptome profiles of tobacco plants under water deficit conditions using microarray technology. In this paper, we describe in detail the experimental procedures and analyses performed in our study. The data set we generated (available in the NCBI/GEO database under GSE67434) has been analysed to identify genes that are involved in the regulation of tobacco's responses to drought.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

RNA-Seq Transcriptome Profiling of Upland Cotton (Gossypium hirsutum L.) Root Tissue under Water-Deficit Stress

An RNA-Seq experiment was performed using field grown well-watered and naturally rain fed cotton plants to identify differentially expressed transcripts under water-deficit stress. Our work constitutes the first application of the newly published diploid D5 Gossypium raimondii sequence in the study of tetraploid AD1 upland cotton RNA-seq transcriptome analysis. A total of 1,530 transcripts were...

متن کامل

بیان فراوان ژن Δ1 – پرولین- 5-کربوکسیلات سنتتاز( p5cs ) ، با هدف افزایش مقاومت به تنش‌های اسموتیک در گیاه تراریخت توتون ( Nicotiana tabacum cv. Xanthi )

Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the c...

متن کامل

بیان فراوان ژن Δ1 – پرولین- 5-کربوکسیلات سنتتاز( p5cs ) ، با هدف افزایش مقاومت به تنش‌های اسموتیک در گیاه تراریخت توتون ( Nicotiana tabacum cv. Xanthi )

Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the c...

متن کامل

Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis

The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might b...

متن کامل

Reactive oxygen species regulate leaf pulvinus abscission zone cell separation in response to water-deficit stress in cassava.

Cassava (Manihot esculenta Crantz) plant resists water-deficit stress by shedding leaves leading to adaptive water-deficit condition. Transcriptomic, physiological, cellular, molecular, metabolic, and transgenic methods were used to study the mechanism of cassava abscission zone (AZ) cell separation under water-deficit stress. Microscopic observation indicated that AZ cell separation initiated ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2015